Combination of fluorescent microscopy and phase contrast microscopy- while a study of a specimen can be done with a fluorescent microscope or with a phase contrast and still obtain a good result, surely a combination of the two methods would more than double the expected results in terms of clarity and vividness. A primer of […]

Phase Contrast Microscopy- A vast spectrum of living biological specimen is virtually transparent when observed in the optical microscope under brightfield illumination. However, to improve visibility and contrast in such specimens, microscopists often reduce the opening size of the sub-stage condenser iris diaphragm, but this maneuver is accompanied by a serious loss of resolution and […]

Preparation of specimen in the phase contrast microscope- One may wonder why specimens are to be prepared in phase contrast microscope when staining is done away with and what is being viewed are living objects or live specimen. However, it is not as simple as that, as most specimens are colorless and transparent. Different procedures […]

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